DLK1/FA1 His Tag Protein, Human,UA BIOSCIENCE_specification/price/image

Product Detail
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Product Specification

Species	Human
Synonyms	Protein delta homolog 1, Pg2, Fetal antigen 1, Pref-1
Accession	P80370-1
Amino Acid Sequence	Ala24-Gln303 with His Tag at the C-terminus
Expression System	HEK293
Molecular Weight	35-43kDa (Reducing)
Purity	＞95% by SDS-PAGE & SEC-HPLC
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, PH7.4, 5% trehalose
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	Mitchell DK, Brewster K, Makri SC, Khan J, Albright EA, Horvai A, Mang H, Lu Q, Dixon SAH, White E, Saadatzadeh MR, Bijangi-Vishehsaraei K, Gampala S, Hickey BE, Leffew H, Li X, Jiang L, Ciesielski MD, Bessler WK, Collier CD, Cohen-Gadol A, Fishel ML, Pratilas CA, Pollok KE, Angus SP, Rhodes S, Clapp DW. DLK1 distinguishes subsets of NF1-associated malignant peripheral nerve sheath tumors with divergent molecular signatures. Clin Cancer Res. 2025 Mar 10.

Background

Human Pref-1 (preadipocyte factor 1), also known as Dlk1 and pG2, is a member of the Notch/Delta/Serrate family of EGF-like repeat-containing proteins that serve as ligands in the Notch signaling pathway. Synthesized as a 383-amino acid precursor with a 23-amino acid signal sequence, a 280-amino acid extracellular region, a 24-amino acid transmembrane segment, and a 56-amino acid cytoplasmic tail, human Pref-1's extracellular region contains six tandem EGF repeats, a juxtamembrane region, and multiple O- and N-glycosylation sites. The 50-60 kDa heterogeneous transmembrane forms of Pref-1 result from variable glycosylation. Mature human Pref-1A shares 85% amino acid identity with mouse and rat homologs. Highly expressed in 3T3-L1 preadipocytes and various endocrine tissues, including pituitary somatotrophs, insulin-producing cells, testicular Leydig cells, and ovarian theca interna and Hilus cells, Pref-1 blocks preadipocyte differentiation into mature adipocytes when constitutively expressed. It also regulates skeletal stem cell, thymocyte, and adrenal gland cell differentiation and inhibits GH secretion in pituitary GH3 cells.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.