Species | Human |
Synonyms | ADORA2A, adenosine A2a receptor, A2aR, ADORA2, RDC8 |
Accession | P29274 |
Concentration | 1.0mg/ml by Nanodrop |
Amino Acid Sequence | Full length with nine mutations, and Flag tag at N-Terminal, and His-Alfa-Twinstrep tag at C-Terminal |
Expression System | HEK293 |
Molecular Weight | 38-45 kDa and 75-95 kDa(Reducing) |
Purity | >90% by SDS-PAGE &>90% by SEC-HPLC |
Endotoxin | <0.1EU/μg |
Conjugation | Unconjugated |
Tags & Cleavage sites | 3C |
Tag | His Tag, Flag Tag, Twinstrep Tag, Alfa Tag |
Physical Appearance | Liquid with detergent |
Storage Buffer | 10 mM Hepes, pH 7.5, 100 mM NaCl, 0. 1 % DDM, 0.01 % CHS. |
Stability & Storage | ·6 months from date of receipt, -60 to -80 °C as supplied. ·about 3 days in 2 to 8 °C. ·Please avoid repeated freeze-thaw cycles. |
Reference | 1. Lebon G, Warne T, Edwards P C, et al. Agonist-bound adenosine A2A receptor structures reveal common features of GPCR activation[J]. Nature, 2011, 474(7352): 521-525. |