Sortase A,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Staphylococcus aureus
Synonyms	LPXTG specific sortase A, sortase, Srt A
Expression System	E.coli
Molecular Weight	25 kDa (Reducing)
Purity	＞98% by SDS-PAGE
Tag	No Tag
Physical Appearance	Liquid
Storage Buffer	50mM Tris-HCl, 100mM NaCl, 2.5mM KCl, pH 8.0, 0.04% Tween 20, 20%甘油
Stability & Storage	Store at -25 ~ -15℃ for 1 years，Avoid freeze / thaw cycle
Reference	1. Frankel B A, Kruger R G , Robinson D E ,et al. Staphylococcus aureus sortase transpeptidase SrtA: insight into the kinetic mechanism and evidence fora reverse protonation catalytic mechanism.[J].Biochemistry, 2005, 44(33):11188-11200. 2. Parthasarathy R, Subramanian S, Boder E T. Sortase A as a Novel Molecular "Stapler" for Sequence-Specific Protein Conjugation[J]. Bioconjugate Chemistry, 2007, 18(2):469-476.

Background

Sortase is a family of transpeptidases found in Gram-positive bacteria, which is divided into two subfamilies: Sortase A and Sortase B. Its main function is to catalyze the covalent attachment of cell surface proteins to the cell wall. Among them, Sortase A (SrtA) has been studied more extensively. Sortase A (SrtA) can specifically recognize proteins with a C-terminal conserved amino acid sequence LPXTG and an N-terminal polyglutamine (X is any amino acid except cysteine and tryptophan).

Components

0.5 ug/ul Sortase A in 50mM Tris-HCl, 100mM NaCl, 2.5mM KCl, 0.04% Tween 20，20%甘油, pH 8.0 @ 25°C
3×Reaction Buffer：100mM Tris-HCl、50mM CaCl2、20mM DTT, pH 7.5 @ 25°C

Protocol

1. Typical polypeptide reaction conditions are as follows:

Components	Volume	Final Concentration
Polypeptide 1 (1 mM)	5 µl	166 uM
Polypeptide 2 (1 mM)	5 µl	166 uM
Sortase A (0.5 ug/ul)	5 µl	-
3X Reaction Buffer	10 µl	1X
ddH2O	Up to 30µl	-

2. After preparation according to the above table, mixed centrifugation, reaction at 30℃ for 1-3h, and SDS-PAGE electrophoresis was performed after reaction.

Guidelines

Avoid freeze / thaw cycle

This product is for research use only.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.