ETAR N-ECD Fc&His Tag Protein, Human,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Human
Synonyms	Endothelin-1 receptor; EDNRA; Endothelin receptor type A; ETA, ETRA; ET-A; ETA-R; hET-AR
Accession	P25101
Amino Acid Sequence	Asp21-Lys80 with Human IgG1 Fc and His Tag at C-Terminus
Expression System	HEK293
Molecular Weight	43-70kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag, Human IgG1 Fc
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, PH7.4, 5% trehalose
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Yanagisawa M, et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988;332(6163):411–415.

Background

Endothelin (ET/EDN) receptors are members of the class A GPCR family and exist in two isoforms: type A (ETAR/EDNRA) and type B (ETBR/EDNRB). These receptors exhibit different affinities for three ligands—ET1, ET2, and ET3. ETAR demonstrates a significantly higher affinity for ET1 and ET2 compared to ET3, while ETBR binds all three ligands nonselectively. The binding affinity rank order for the ET-A receptor is: ET1 > ET2 >> ET3.
ETAR mediates its action by associating with G proteins, which activate a phosphatidylinositol-calcium second messenger system. Both angiotensin II receptor type 1 (AT1R) and endothelin-1 receptor type A (ETAR) are G-protein-coupled receptors (GPCRs) found on the surface of various cell types, including immune cells, vascular smooth muscle cells, endothelial cells, and fibroblasts. These receptors are activated by endothelin 1 (ET1) and angiotensin II (AngII), respectively.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.