DT3C (Diphtheria toxin & spg 3C domain) Protein, Corynephage beta,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Synonyms	DT3C, Diphtheria toxin & spg 3C domain
Accession	P00588、P19909
Amino Acid Sequence	Gly33-Lys417&&ILe291-Glu497
Expression System	E.coli
Molecular Weight	65kDa (Reducing)
Purity	＞90% by SDS-PAGE and HPLC
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	20mM Tris, 200mM NaCl, pH8.0
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	·12 months from date of receipt, -20 to -70 °C as supplied. ·1 month, 2 to 8 °C under sterile conditions after reconstitution. ·Please avoid repeated freeze-thaw cycles.
Reference	Biochem Biophys Res Commun. 2014 Nov 28; 454(4):600-3.Epub 2014 Nov 1.

Background

DT3C consists of diphtheria toxin (DT) lacking the receptor-binding domain but containing the C1, C2, and C3 domains of Streptococcus protein G (3C). When a mAb–DT3C conjugate, which functions in vitro like an ADC, reduces the viability of cancer cells, the mAb being tested must have been internalized by the target cells. DT3C can thus be a tool to identify efficiently and easily mAbs that can be internalized by cells, thereby enhancing the development of promising ADCs.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.