Recombinant Carboxypeptidase B，Bovine,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Bovine
Synonyms	Carboxypeptidase B
Expression System	PichiaPastoris
Molecular Weight	33 kD
Purity	＞95% by SDS-PAGE & HPLC
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	20mM Tris-HCl 、100mM NaCl
Reconstitution	Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg /ml.
Stability & Storage	·24 month, 2 to 8 °C under sterile conditions . After dissolution, store at -20℃ and avoid repeated freeze-thaw cycles.
Reference	[1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40. [2] James W. Brodrick, Michael C. Geokas, Corey Largman, Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion, Biochimica et Biophysica Acta (BBA) - Enzymology, 1976, 468-481.

Background

Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.

Components

20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B

Protocol

The recommended amount of Carboxypeptidase B is 1:50~1:1000 (w/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.

Guidelines

1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.

2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.

Unit Definition

The amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.