Ribonuclease R (RNase R) is a Mg2+ dependent 3'→5' exonuclease derived from Escherichia coli. RNase R can digest all linear RNA. However, it cannot digest ring-shaped RNA, lasso structure or double-stranded RNA molecules with 3 'end protruding ends less than 7 nucleotides, tRNA and 5SRNA with complex secondary structure. RNase R is commonly used in gene expression and variable shear studies and can digest linear RNA to enrich circular RNA or lasso structured RNA. This product does not contain DNase, other RNA endonuclease and exonuclease activities.
Storage Solution: 20U/μl RNase R, 50mM Tris-HCl, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100 (pH7.5 @25℃)
10*Reaction Buffer: 200mM Tris-HCl, 1M KCl, 1mM MgCl2 (pH8.0 @25℃)
1. The activity of RNase R requires 0.1-1.0 mM Mg2+;
2. With the increase of substrate RNA, digestion time and enzyme amount can be appropriately prolonged
One unit converts 1μg of poly-r(A) into acid-soluble nucleotides in 10 minutes at 37℃ in 20mM Tris-HCl (pH8.0), 100mM KCl and 0.1mM MgCl2
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