IL-2(Cys145Ser) Protein, Human,UA BIOSCIENCE_specification/price/image

Product Detail
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Product Specification

Species	Human
Synonyms	IL-2(Cys145Ser), IL-2 C145S
Accession	P60568
Amino Acid Sequence	Ala21-Thr153(Cys145Ser)
Expression System	E.coli
Molecular Weight	15kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	No Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Pol, J. G. , Caudana, P. , Paillet, J. , Piaggio, E. , & Kroemer, G. . (2020). Effects of interleukin-2 in immunostimulation and immunosuppression. The Journal of Experimental Medicine(1), 217. 2.Kolios, A. G., Tsokos, G. C., & Klatzmann, D. (2021). Interleukin-2 and regulatory T cells in rheumatic diseases. Nature Reviews Rheumatology, 17(12), 749-766. 3.Zhang, Y., & Su, J. (2023). Interleukin-2 family cytokines: An overview of genes, expression, signaling and functional roles in teleost. Developmental & Comparative Immunology, 104645.

Background

IL-2(Cys145Ser) is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. It has been engineered to contain the serine for cysteine substitution at amino acid position 145. IL-2(Cys145Ser) has similar characteristics and functions to IL-2, which is a leukocytotrophic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. Mature human IL-2 shares 56% and 66% amino acid (aa) sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells, and plays a vital role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity. Recombinant IL-2 is widely used in cell culture for the expansion of T cells.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.