HRV 3C Protease,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Synonyms	Protease 3C、PreScission Protease
Amino Acid Sequence	/
Expression System	E.coli
Molecular Weight	21 kD (Reducing)
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v/v) glycerol，（pH 8.0 @ 25℃）
Reconstitution	/
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Knott J A , Orr D C , Montgomery D S ,et al.The expression and purification of human rhinovirus protease 3C.[J].European Journal of Biochemistry, 1989, 182(3):547-55. [2] Xu H , Wang Q , Zhang Z ,et al.A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease[J].Enzyme and Microbial Technology, 2019, 123:15-20.

Background

Recombinant human rhinovirus(HRV)3C protease is widely used in the purification and productions of proteins due to its good stability，high specificity，and enzymatic activity．3C protease is one of the non—structural proteins of HRV，and plays important roles in the life cycle of HRV．This protease specifically recognizes the sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, and cleaves the peptide bond between Gln and Gly.

Components

Storage Solution: 1U/μl 3C Protease50mM Tris-HCl，150mM NaCl，10mM EDTA，1mM DTT，50%(v/v) glycerol（pH 8.0 @ 25℃） 10*Reaction Buffer: 500mM Tris-HCl，1.5M NaCl，10mM EDTA，10mM DTT，（pH 7.5@ 25℃）

Protocol

1. Combine 100 μg of fusion protein, 10 μl of 10*Reaction Bufferr and H20 (if necessary) to make a 100 μl total reaction volume. 2. Add 1 µl HRV 3C Protease 3. Incubate reaction at 5°C for 16 hour.

Guidelines

Please avoid repeated freeze-thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to remove > 90% of the GST label from 100 µg of fusion protein at 5°C for16 hour.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.