Enterokinase, Bovine,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Bovine
Synonyms	rBoEnterokinase; Enteropeptidase; ENTK; PRSS7,
Expression System	E.coli
Molecular Weight	28 kDa (Reducing)
Purity	＞95% by SDS-PAGE and HPLC
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, pH 7.4 @ 25°C
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	1.Yong S Z, Yong C J, Yuan C X, et al. Secretory Expression, Purification and Characterization of Bovine Enterokinase Light Chain[J]. Journal of Nanjing University (Natural Sciences), 2004. 2.Haidong, Tan, and, et al. Purification and refolding optimization of recombinant bovine enterokinase light chain overexpressed in Escherichia coli[J]. Protein Expression & Purification, 2007.

Background

Enterokinase (enteropeptidase, EC 3.4.4.8) occupies a key position in the utilization of dietary proteins. The enzyme initiates intraluminar digestion of proteins by the proteolytic conversion of trypsinogen to trypsin, which in turn activates the other pancreatic zymogens (Kunitz, 1939a,b; Hadorn et al., 1969). The proteolytic attack of enterokinase is directed exclusively toward the Lys6-Ile7 peptide bond of trypsinogen leaving all other lysine and arginine bonds in the molecule unaffected (Maroux et al., 1971). The resultant cleavage produces the simultaneous release of active trypsin and of the
amino-terminal hexapeptide Val-(Asp)d-Lys (Rovery et al., 1953; Davie and Neurath, 1955). This unique specificity exhibited by enterokinase is of interest as it relates to both the molecular basis of substrate recognition and the control of the digestive process.

Components

5 U/μL Enterokinase, Bovine in 20 mM Tris-HCl (pH 7.4 @ 25°C), 200 mM NaCl, 2 mM CaCl2 and 50% (v/v) glycerol

Protocol

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
1. Combine 500 ug of sample with reaction buffer
* Recommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)
2. Add 1 U of Enterokinase light chain
3. Incubate at 25°C for 16 hours

Guidelines

1. Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50 mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 500 µg of substrate to 95% completion in 16 hours at 25°C.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.