VHL/Elongin-C/Elongin-B Complex Protein, Human,UA BIOSCIENCE_specification/price/image

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Product Specification

Species	Human
Antigen	ELOB/ELOC/VHL
Synonyms	Elongin B & Elongin C & VHL, ELOB & ELOC & VHL Complex, Elongin B & Elongin C & VHL Complex
Accession	Q15370、Q15369、P40337
Amino Acid Sequence	ELOB flag tag, Human, Asp2-Gln118, N-flag DYKDDDDKDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVQ ELOC flag tag, Human, Asp2-Cys112, N-flag DYKDDDDKDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC VHL His tag, Human, Pro2-Asp213, N-His HHHHHHHHHHGGGSGGGSPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD
Expression System	Baculovirus-InsectCells
Molecular Weight	14 kDa (ElOB) and 13.3 kDa (ElOC) and 25.9 kDa (VHL)
Purity	>95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	40mM Tris, 200mM NaCl, 2.2mM KCl, 20% glycerol, 3mM DTT, pH8.0
Reconstitution	N.A
Stability & Storage	12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles.
Reference	1.Cell Chem Biol. 2023 Jul 20;30(7):766-779.e11. Epub 2023 Jun 23. 2.bioRxiv. 2023 May 24;2023.05.22.541439. PreprintOkumura F., et al. (2012) Front. Oncol. 3.Mod Pathol. 2023 Apr 23;36(8):100194. Online ahead of print.

Background

ELOB (elongin B) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. ELOB and ELOC(elongin C) form a heterodimer that serves as the regulatory subunit for the Elongin complex-a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites. The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. The VHL(von Hippel-Lindau disease tumor suppressor) binds to ELOB and ELOC and thereby inhibits transcription elongation.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.