H1N1 NP (A/Puerto Rico/8/1934) His Tag Protein, Influenza,UA BIOSCIENCE_specification/price/image

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Product Specification

Species	Influenza
Antigen	NP/Nucleoprotein
Synonyms	Nucleoprotein, Nucleocapsid protein, NP.
Accession	Accession#: P03466
Amino Acid Sequence	Met1-Asn498, with C-terminal 10*His MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRNPGNAEFEDLTFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGTKVLPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDRTTIMAAFNGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFGDNAEEYDNGGGSGGGSHHHHHHHHHH.
Expression System	Hi5(Baculovirus-InsectCells)
Molecular Weight	57-60 kDa
Purity	＞95% by SDS-PAGE and HPLC
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	1*PBS, 1mM DTT, 1mM EDTA, pH7.4
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Pang B, Cheung NN, Zhang W, Dai J, Kao RY, Zhang H, Hao Q. Structural Characterization of H1N1 Nucleoprotein-Nucleozin Binding Sites. Sci Rep. 2016 Jul 11;6:29684. 2.Ortega J, Martín-Benito J, Zürcher T, Valpuesta JM, Carrascosa JL, Ortín J. Ultrastructural and functional analyses of recombinant influenza virus ribonucleoproteins suggest dimerization of nucleoprotein during virus amplification. J Virol. 2000 Jan;74(1):156-63.

Background

Nucleoprotein, encoded by influenza A virus, is an attractive target due to its high conservative and multifunctional nature during virus life cycle. In addition, NP does not have any counterpart in the cell, and hence compounds targeting NP might have less off-target side effects. NP encapsulates the negative strand viral RNA by is binding to 24 bases of RNA, protecting it from nucleases. The encapsulated genomic RNA is termed the ribonucleoprotein (RNP). The active RNP imports into the nucleus through cellular importin α/β pathways, and serves as template for transcription and replication.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.