Biotinylated Fc γ RIIA/CD32a(R167) His&Avi Tag Protein, Human,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Human
Synonyms	CD32a, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2
Accession	P12318-1
Amino Acid Sequence	Ala36-Ile218, with C-terminal His and Avi Tag
Expression System	HEK293
Molecular Weight	30-35kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Biotin
Tag	Avi Tag, His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4.
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.Benjamin Descours, Gaël Petitjean, José-Luis López-Zaragoza, Timothée Bruel, Raoul Raffel, Christina Psomas, Jacques Reynes, Christine Lacabaratz, Yves Levy, Olivier Schwartz, Jean Daniel Lelievre & Monsef Benkirane: CD32a is a marker of a CD4 T-cell HIV reservoir harbouring replication-competent proviruses, Nature volume 543, pages564–567 (2017)..

Background

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human Fc gamma Rs are classified into three classes: RI (CD64), RII (CD32), and RIII (CD16), which give rise to multiple isoforms (1-3). Fc gamma RI is a high-affinity receptor that binds monomeric IgG, and Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complex IgG (IC). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence identity with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types: macrophages, neutrophils, eosinophils, platelets, dendritic cells, and Langerhans cells, where inhibitory ITIM receptors may also be co-expressed and co-engaged by specific ligands. Signaling through FcγRIIA leads to the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation, and cytokine production) that can be modulated by signals from inhibitory receptors. The strength of the signal depends on the ratio of activated and inhibitory receptor expression.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.