Pyrophosphatase, Inorganic（无机焦磷酸酶）,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Yeast
Synonyms	Inorganic pyrophosphatase、PPase
Concentration	0.1U/μl
Expression System	E.coli
Molecular Weight	33.5 kD
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C)
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Lahti R , Pitk Ranta T , Valve E ,et al. Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12.[J].Journal of Bacteriology, 1988, 170(12):5901-7 [2] Satoh T , Watanabe M , Nogi S I ,et al. Molecular Cloning, Expression, and Site-Directed Mutagenesis of Inorganic Pyrophosphatase from Thermus thermophilus HB8[J]. Journal of Biochemistry, 1998, 124(1):79-88.

Background

Pyrophosphatase, Inorganic is a yeast derived inorganic pyrophosphatase (PPase) expressed by Escherichia coli, which is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two molecules of phosphate ions, causing the inorganic pyrophosphate hydrolysis to form phosphates. In molecular biology, it can be used to increase the production of RNA in vitro transcription reactions.

Components

Storage Solution: 0.1U/μl Pyrophosphatase, Inorganic、 20 mM Tris-HCl、100 mM NaCl、1 mM DTT、0.1 mM EDTA 50% Glycerol (pH 8.0 @ 25°C)

Protocol

Use 1-3 units per ml in a high yield in vitro RNA synthesis reaction

Unit Definition

One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2 and 2 mM PPi).

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.