Endo S,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Streptococcus pyogenes
Synonyms	Endo-beta-N-acetylglucosaminidase EndoS, Endoglycosidase S, EndoS
Expression System	E.coli
Molecular Weight	109 kDa (Reducing)
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C）
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Collin,M.EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG[J].Embo Journal, 2014, 20(12):3046-3055. [2] Beatriz,Trastoy,Joseph,et al.Liquid–liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on human IgG[J].Acta Crystallographica, 2013.

Background

Anenzyme expressed by Streptococcus pyogenes capable of releasing the terminal sialic acid residues from glycoproteins such as immunoglobulins.Specifically catalyzes the hydrolysis of the beta-1,4 linkage between the first two N-acetylglucosamine residues of the complex-type N-linked glycan located on 'Asn-297' of the Fc region of IgG antibodies (IGHG1, IGHG2, IGHG3 or IGHG4), thereby preventing interaction between IgGs and Fc receptors and ability to activate the complement pathway. Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.

Components

Storage Solution : 200U/ul Endo S、20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. (pH 7.5 @ 25°C)

10*Reaction Buffer：50mM CaCl2、500 mM sodium acetate (pH 5.5 @ 25°C)

Protocol

1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume.

2. Add 1 µl Endo S.

3. Incubate reaction at 37°C for 1 hour

Unit Definition

One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10µl.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.