DKK-1 Protein, Human,UA BIOSCIENCE_specification/price/image

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Product Specification

Species	Human
Synonyms	Dickkopf-1, Dkk-1, hDkk-1, DKK1
Accession	O94907
Amino Acid Sequence	Thr32-His266, with N-terminal His Tag
Expression System	HEK293
Molecular Weight	37-46kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4.
Reconstitution	Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1. Fedi P, Bafico A, Soria AN, Burgess WH, Miki T, Bottaro DP, et al. Isolation and Biochemical Characterization of the Human Dkk-1 Homologue, a Novel Inhibitor of Mammalian Wnt Signaling. J Biol Chem (1999) 274(27):19465–72. 10.1074/jbc.274.27.19465 2. Hameed A, Brady JJ, Dowling P, Clynes M, O’Gorman P. Bone Disease in Multiple Myeloma: Pathophysiology and Management. Cancer Growth Metastasis (2014) 7:33–42. 10.4137/CGM.S16817 3.Pinzone JJ, Hall BM, Thudi NK, Vonau M, Qiang YW, Rosol TJ, et al. The Role of Dickkopf-1 in Bone Development, Homeostasis, and Disease. Blood (2009) 113(3):517–25. 10.1182/blood-2008-03-145169

Background

DKK-1 is a 266-amino acid protein with an approximate molecular mass of 26-kDa, and it possesses six secondary structures, which are two alpha helices and four β-sheets. It has the colipase-like fold in C-terminal Cys domain, which likely allows it to serve as an interactive surface. DKK-1 expression appears to be preferential in some adult tissues including bone, placenta, prostate, spleen, and colon. DKK-1 has been shown to play an important role as a Wnt signaling pathway antagonist in several studies. DKK-1 is down-regulated in human colon tumours; hence, it may act as a tumour suppressor. High DKK-1 expression indicated favourable responses to chemotherapy in brain tumours whereas; over-expression of DKK-1 was found in human hepatoblastomas, Wilms’ tumours and multiple myelomas. Moreover, since overexpression of DKK-1 in osteoblasts causes osteopenia, it is also regarded as a negative regulator of normal bone homeostasis.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.