| Usage |
Ready-to-use, no dilution required. Antibody elution: Preheat the antibody eluate at 37 °C, add a small amount of antibody eluate dropwise to cover the sample, and place it at room temperature for 3 to 5 minutes; Discard the eluate, add a sufficient amount of eluate dropwise again to cover the sample (the amount of eluate should be slightly bulged on the film, generally 150 ~ 300μL), 37 °C for 10 ~ 40min (generally 20min is enough); PBS wash 3 times (fixative can be added to briefly fix again). |
| General Notes |
1. The effect of antibody elution depends on the thickness of the section, elution temperature and time, and the type of primary antibody (elution difficulty: structural membrane protein and cytoskeletal protein > cytoplasmic protein > nuclear protein). 4-8 μm slices can usually be treated in a wet box at 37 °C for 10-40 minutes.
2. Some antibodies, such as E-cadherin and Tuj1, bind to the antigen very strongly, and the elution temperature can be increased to 50 °C, or the elution time can be extended to 60min.
3. If there is a problem with the elution of some antibodies, you can reduce the dilution ratio of the primary antibody, or put these antibodies in the last round of TSA labeling.
4. Appropriate protective measures should be taken to avoid contact between the reagent and the skin and eyes. If you accidentally come into contact with sensitive areas, rinse immediately with plenty of water.
5. The activity of reagents beyond the validity period may be reduced, so please use them within the validity period of the reagent. |
| Storage Temp. |
Stored at 2 ~ 8 ℃, the validity period is 12 months. |
| Applications |
The antibody eluate is used for paraffin sections, frozen sections, bone tissue sections, cell crawling sections and other samples, and the antibody elution during TSA staining. |
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