Product introduction

This product provides a unique lysis buffer system to quickly release RNA from cultured cell samples for RT-qPCR reactions, eliminating the time-consuming and laborious RNA purification process, and only takes 7 minutes to obtain the required RNA template. The 5× Direct RT Mix and 2× Direct qPCR Mix-SYBR provided in the box can quickly and effectively obtain real-time quantitative PCR results.

5× Direct RT Mix and 2× Direct qPCR Mix-SYBR have strong inhibitor tolerance, and can use the lysate of the sample to be tested as a template for efficient reverse transcription and specific amplification. The reagent contains Foregene unique Reverse Transcriptase with high affinity for RNA , as well as Hot D- Taq DNA Polymerase , dNTPs, MgCl₂ , reaction buffer, PCR optimizer and stabilizer , and can be used in conjunction with the lysis buffer to detect the sample quickly, easily and accurately, and it has the characteristics of high sensitivity, strong specificity and good stability.

The kit is aimed at micro-system lysis of 96-well cultured cells, and has good uniformity and consistency; the kit components provide 96 lysis reactions, 96 reverse transcription reactions and 96×2 qPCR reactions, which can meet the 96-well cells plate for one-time use, avoiding the pollution caused by repeated opening, freezing and thawing of reagents and the degradation of reagent performance.

Specification

 *: The lysis reagent DNA Eraser is included in Part II of the kit; Cell Lysis, RT, and qPCR components can be purchased separately.

Transport and storage conditions

Transportation conditions: The kits should be transported in a low-temperature ice box to ensure that the kit is in a state of <4 °C .

Storage conditions : Kit Part I should be stored at 2-8 °C ; Part II should be stored at -20± 5 °C .

Precautions: (Please be sure to read the precautions carefully before using the kit)

- Pay attention to the operation method of the experiment to avoid cross-contamination between samples.

- Pay attention to the cleanliness of the experimental environment and utensils to avoid RNase contamination, which will cause RNA degradation.

- The cell lysis system should be freshly prepared and ready to use .

- 2× Direct qPCR Mix-SYBR should avoid repeated freezing and thawing, otherwise the PCR efficiency will be affected.