Description:
This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.
5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.Use in conjunction with lysis buffer to detect samples quickly and easily, with high sensitivity, specificity, and stability.
Specification
|
Kit composition
(20 μl qPCR reaction system)
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DRT-01011
|
DRT-01012
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Component
|
|
200 T
|
1000 T
|
|
Part I
|
Buffer CL
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4 ml
|
20 ml
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Cell Lysis
|
|
Foregene Protease Plus II
|
80 μl
|
400 μl
|
|
Buffer ST
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400 μl
|
1 ml × 2
|
|
Part II
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DNA Eraser
|
80 μl
|
400 μl
|
|
5× Direct RT Mix
|
160 μl
|
800 μl
|
RT
|
|
2× Direct qPCR Mix-SYBR
|
1 ml × 2
|
1.7 ml × 6
|
qPCR
|
|
50× ROX Reference Dye
|
40 μl
|
200 μl
|
|
RNase-Free ddH2O
|
1.7 ml
|
10 ml
|
-
|
|
Manual
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1 serving
|
1 serving
|
1 serving
|
*: Cell Lysis, RT and qPCR components can be purchased separately. For details, see Appendix 1 of the full version of the manual (PAGE 13).
Features
- Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.
- The sample demand is small, as low as 10 cells can be tested.
- High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.
- DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.
- Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.
Kit application
- Scope of application: cultured cells.
- RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.
- The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.
Transport and storage conditions
Transportation conditions: The whole process is transported in a low-temperature ice box to ensure that the kit is in a state of <4 °C.
Storage conditions: Part I of the kit is stored at 4 °C; Part II is stored at -20°C.
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