Description:

This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× PCR Easy^TM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Specification

-No time-consuming and expensive DNA purification

-The sample demand is small, as little as 5mg animal tissue can be tested.

-No special treatment such as grinding and crushing is required, and the operation is simple.

-Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Kit application

-Scope of application: a variety of animal tissues.

-DNA released from sample lysis: Use as PCR template only.

-The kit can be used for the following purposes: identification of transgenes, animal genotyping, etc.

Storage conditions

1. Shipping Conditions

The whole process is transported in a low temperature ice box to ensure that the Part I and Part II of the kit are in a state of <4°C.

2. Storage conditions

-This kit Part I is stored at room temperature or 2-8℃.

-Reagent Buffer AL can be stored for 12 months under dry conditions; for longer storage, it can be stored at 2–8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Be sure to place the solution in the kit at room temperature for a period of time before use, and if necessary, preheat in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix well before use.

-Reagent Foregene Protease has a unique formula, in order to ensure its better activity and stability, please store it at 4℃.

-Reagent 6×DNA Loading Buffer, can be stored at 4°C or -20°C for a long time.

This kit Part II is stored at -20°C.

-Reagent 2×PCR Easy^TM Mix, if used frequently, it can also be stored at 4°C for a short time (limited to be used up within 10 days).