Product Description:

  The iSR Taq DNA polymerase is a cloned heat-stable DNA polymerase from Thermus aquaticus. The DNA polymerase gene is modified by site-directed mutagenesis and then expressed in Escherichia coli. We use the Sustained Release Technology (SRT) to produce the iSR Taq DNA Polymerase on a large scale. The sustained release side chain is covalently attached to the polymerase to form a liposome-like structure, which encapsulates the polymerase so that the polymerase has extremely high stability and hot start activity.

Product Specifications:

  5000U, 50,000U, customizable.
    Concentration: 5 units/μl (5U/μl).

Transportation and Storage:

  -25℃ ~ -15℃ 

Product Number:

  Taq DNA polymerase storage buffer:

No.		Main component	Applications
iT1001  Normal type		20mM Tris, 100mM KCl, 0.1% CA-630, 30% glycerol, 0.01% NaN3, pH=7.8～8.0	Routine use,  Liquid PCR reagent
iT1002  Freeze-dried type		20mM Tris, 100mM KCl, 0.01%, NaN3, ProClin 300, pH=7.8～8.0	Suitable for lyophilized PCR reagents
iT1003  Drying type		20mM Tris, 100mM KCl, 0.01%, NaN3, ProClin 300, 0.2% PVA, pH=7.8～8.0	Suitable for drying PCR reagents

  10×Buffer:

No.	Main Component	Applications
10×Buffer #1 Normal Type	Tris-HCl 200mM, MgCl2 25mM, KCl 750mM, NaN3 0.01%, Gelatin 0.2%, pH=8.3	Routine use  Liquid PCR reagents, etc.
10×Buffer #2 Normal Type	Tricine 200mM, Mg(OAc)2 25mM, KOAc 750mM, NaN3 0.01%, Gelatin 0.2%, pH=8.3	Conventional PCR  One-step reverse transcription PCR
10×Buffer #3     Drying type	Tricine 200mM, Mg(OAc)2 25mM, KOAc 750mM, NaN3 0.01%, Gelatin 0.2%, PVA, etc. pH=8.3	Conventional PCR  Drying PCR MIX
10×Buffer #4 Freeze-dried type	Tricine 200mM, Mg(OAc)2 25mM, KOAc 750mM, NaN3 0.01%, Gelatin 0.2%, PVA , antistatic agent, etc. pH=8.3	Conventional PCR,  Lyophilized PCR MIX, etc.
2×Buffer #5 Freeze-dried type	Mannitol, rhamnose, pullulan, polymers, etc.	PCR MIX freeze-dried skeleton

Activity and Purity:

  One U (unit): Using activated salmon sperm DNA as template/primer, at 74℃, within 30 minutes, the amount of enzyme that can incorporate 10 nmols of total dNTPs into an acid-insoluble material is defined as one unit (U).

  Purity: no nuclease pollution, no exonuclease pollution, purity>95%.

Feature of Product:

  Long half-life: the effective enzyme activity in the amplification process is constant and does not attenuate by using the sustained release technology.

 Strong anti-interference ability: sustained release technology reduces the immunogenic activity of Taq DNA Polymerase and improves the anti-interference ability of the Taq DNA Polymerase.

 High Fidelity: the fidelity of the Taq DNA Polymerase can be greatly improved by reducing the charge repulsion in PCR. 

  The best choice for microfluidics - an excellent partner for dry-reagent-based PCR:

Dispensing-drying: drying at 37°C or 50°C, stability of iSR Taq up to 12 months;

Freeze-drying: iSR Taq has a wider freeze-drying curve and a simpler process. It can be combined onto the backbone of excipients such as rhamnose, mannitol, glucose, sucrose, etc.

Applications:

  TaqMan probe-based real-time PCR analysis: iSR Taq has 5'~3' exonuclease activity and can hydrolyze the hybridized TaqMan probes.

  TA cloning: No Proofreading activity (3'→5' exonuclease activity), suitable for TA cloning.

  Multiplex real-time PCR: suitable for real-time PCR of less than five colors.

  Gene mutation and SNP detection: suitable for detecting gene mutation and SNP (using allele or ARMS method).

Taq DNA Polymerase Protocols:

  General composition of PCR reaction mixture (total 50 μl):

  Real-Time PCR reaction conditions:

Precautions:

  Before use, please read the instructions carefully and conduct experiments in strict accordance with the instructions. If you need help, please contact technical support.

  The iSR Taq may contain traces of E. coli genomic DNA, so it is not recommended for detection of E. coli or other bacteria. To amplify the E. coli genome, please use a DNA/RNA-Free Taq DNA Polymerase.

  If it is applied to one-step real-time PCR, please select an appropriate PCR Buffer.

  When using iSR Taq to detect bacteria and viruses, please try to avoid mismatches at the 3' end of primers. Single-base mutation or single nucleotide polymorphisms that occur in primer binding sites will cause the Ct value to be 6-12 cycles later than expected Ct value.

 Attachment 1: iSR Taq DNA Polymerase purity and performance application examples

 ★ The iSR Taq DNA polymerase adopts a brand-new production process and low-temperature purification processes, multiple purification cycles to ensure the purity and stability of the enzyme to the greatest extent. The purity of the iSR Taq DNA Polymerase >95% .