1. Principle
Florfenicol ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Florfenicol in milk, honey, tissue ans serum.
The coupling antigens are coated on the micro-well stripes. The Florfenicol in the sampleand the coupling antigens coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Florfenicol in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Florfenicol concentration in the sample.
2. Technical specification:
Sensitivity: 0.10ppb Detect time: Approx.45 min
Detect limit:
Chicken, duck, pork, shrimp, fish ........ 0.30ppb
Honey......................................................... 0.30 ppb
Feed..............................................................1.20 ppb
Serum and milk ........................................2.0 ppb
Cross-reaction rate:
Florfenicol.................................................. 100%
Florfenicol Amine .......................................15%
Chloramphenicol.......................................<1%
Thiamphenicol.......................................... <1%
Wuhan Ecalbio Co., Ltd
Web: www.ecalbio.com
Address: HZAU, Nanhu Road, Wuhan city 430075, China.
Tel: +86 27 87745856
Fax: +86 27 87745856
E-mail: info@ecalbio.com
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