1. Principle
Imidacloprid ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Imidacloprid in the fruit, plant isolate, vegetable and tea samples.
The coupling antigens are coated on the micro-well stripes. The Imidacloprid in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Imidacloprid antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Imidacloprid in it. The measurement is made photometrically at 450nm. The absorption is inversely proportional to the Imidacloprid concentration in the sample.
2. Technical specification
Sensitivity: 0.20ppb
Detect time: Approx.75 min
Cottonseed or cotton isolate:
Fruit, vegetable and tea...........3.0ppb
Cross-reaction rate:
Imidacloprid..........................................100%
Recovery rate:
Fruit, vegetable and te................80±20%
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