| Cell Line Description |
The H9c2 (2–1) myoblast cell line, isolated from ventricular tissue, is currently used in vitro as a mimetic for skeletal and cardiac muscle due its biochemical, morphological and electrical/hormonal signaling properties. The H9c2 cell line was originally isolated from the ventricular portion of the BDIX rat heart. Thirteen days after fertilization, the cells were isolated and immortalized. An important feature of this embryonic cell line is its ability to differentiate from mononuclear myoblasts into myotubes, acquiring an elongated shape and orienting in a parallel manner, when cultured in a medium with low serum concentrations. During differentiation, the cells primarily acquire a skeletal muscle phenotype, as evidenced by cell type-specific differentiation markers such as myogenin and MyoD. |
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