Glycosylation is an important post-translational modification of protein. The types of glycosylation of protein can be classified into N-glycosylation and O-glycosylation, depending on the types of connection between the peptide and sugar. N- glycosylation is connected by the N- acetylglucosamine (Glc-NAc) at the reduced end of the sugar chain and the nitrogen atoms on some of the Asn side chain amino groups in the peptide chain. The Asn connected to the sugar chain must be in the motif made up of Asn-X-Ser/Thr 3 residues, in which X is an arbitrary amino acid residue with the exception of Pro. The structure of O-glycosylation is simpler than N-glycosylation, and the sugar chain is short, but with much more diversities. O-glycosylated peptides are mainly linked to sugar via Ser and Thr residues, however, some types of O-glycosylation may be connected by tyrosine, hydroxyllysine and hydroxyproline residues. MtoZ Biolabs uses Obitrap Fusion Lumos mass spectrometry and Byonic software for glycan profiling analysis of the peptides, protein and antibody drugs. The system has been optimized for determination of the composition of glycosylation and the sites of N-O-glycosylation of peptides or protein.
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MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.
MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.
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Email: marketing@mtoz-biolabs.com
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https://www.mtoz-biolabs.com
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