REAGEN™ Ammonia Assay Kit is a cuvette-based enzymatic assay for the determination of Ammonia across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Ammonia directly from food samples.
The unique features of the kit are:
- High sensitivity and low detection limit (0.2 ppm)
- High reproducibility
- A rapid (30 minute), robust enzyme-based assay
Procedure Overview
REAGEN™ Ammonia Assay Kit uses a coupled enzymatic reaction scheme to detect Ammonia in food samples. The ammonia is used as a substrate by the enzyme glutamate dehydrogenase to make glutamate. In the process, NADH is stoichiometrically converted to NAD+. This conversion, measured at 340 nm, is proportional to the level of Ammonia in the sample.
The absorbance of each sample at 340 nm is measured using a spectrophotometer. The concentration of Ammonia in each sample is then directly determined from the change in NADH absorbance at 340 nm within 25 minutes.
Sensitivity (Detection Limit)
|
Sample Volume
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Detection Limit (mg/L)
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0.1 mL
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4
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2 mL
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0.2
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The linear range of the assay is 0.6 – 600 mg/L (ppm).
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