Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit,Abbkine_specification/price/image

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NFE2-binding sites and Maf recognition elements (MAREs) are essential cis-acting elements in the regulatory regions of erythroid-specific genes recognized by the erythroid transcription factor NFE2, which is composed of p45 and a small MAF protein. The p45 subunit and the p45-related factors NRF1 (NFE2L1) and NRF2 (NFE2L2) contain a Cap'n'collar (CNC)-type basic leucine zipper (bZIP) domain, and they are collectively grouped as the CNC family. The cDNA sequence encodes a novel 694-amino acid polypeptide, which the authors called NRF3. NRF3 contains a bZIP domain that is similar to those of other CNC transcription factors. Northern blot analysis detected 2 human NRF3 transcripts in all tissues examined, with the highest levels in placenta. NRF3 was expressed in B- and monocytic cell lines but not in T-cell lines.

Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit employs a two-site sandwich ELISA to quantitate NFE2L3 in samples. An antibody specific for NFE2L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNFE2L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFE2L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFE2L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Nuclear factor erythroid 2-related factor 3 (NFE2L3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.