The PRODH gene provides instructions for producing the enzyme proline oxidase (also known as proline dehydrogenase), which is found primarily in the brain, liver, and kidney. Within cells of these organs, this enzyme functions in energy-producing structures called mitochondria. Proline oxidase begins the process of breaking down the protein building block (amino acid) proline by starting the reaction that converts it to pyrroline-5-carboxylate. A subsequent step converts this intermediate product to the amino acid glutamate. The conversion between proline and glutamate is important in maintaining a supply of the amino acids needed for protein production, and for energy transfer within the cell.
Mouse Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH in samples. An antibody specific for PRODH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRODH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-proline-dehydrogenase-1-mitochondrial-prodh-elisa-kit-kte70655/
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