SYT12 is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. Studies of the orthologous gene in rat have shown that the encoded protein selectively modulates spontaneous synaptic-vesicle exocytosis and may also be involved in regulating calcium independent secretion in nonneuronal cells. Alternative splicing results in multiple transcript variants. SYT12 has previously been referred to as synaptotagmin XI but has been renamed synaptotagmin XII to be standard with mouse and rat official nomenclature.
Mouse Synaptotagmin-12 (SYT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT12 in samples. An antibody specific for SYT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Synaptotagmin-12 (SYT12) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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