Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit,Abbkine_specification/price/image

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Using database analysis and cDNA sequencing from a human caput library, Hamil et al. (2003) identified LCN6. The 163-amino acid LCN6 protein contains a predicted 20-amino acid signal peptide.human LCN6 is 93% identical to rhesus monkey Lcn6 and lacks a second, C-terminal cysteine found in many lipocalins. The LCN6 protein contains multiple phosphorylation sites, and structural prediction programs showed that LCN6 displays the characteristic lipocalin 3-dimensional structure. Northern blot analysis revealed robust expression of a 1-kb LCN6 transcript in epididymis. Using immunohistochemistry, strong expression of LCN6 protein in the caput epithelium and lumen; immunofluorescence of human spermatozoa showed strong LCN6 expression in large patches in the postacrosomal region of the head.

Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit employs a two-site sandwich ELISA to quantitate LCN6 in samples. An antibody specific for LCN6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCN6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCN6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCN6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Epididymal-specific lipocalin-6 (LCN6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.