Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit,Abbkine_specification/price/image

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Dermcidin is a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients.High performance liquid chromatography, mass spectrometry, and microsequence analysis of sweat showed a processed 47-amino acid DCD peptide with a calculated mass of 4.7 kD, compared with the expected 9.3 kD of full-length DCD without the signal peptide.DCD expression in skin, melanocytic nevus, and cutaneous melanoma tissue, but not in any other organ systems tested.

Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit employs a two-site sandwich ELISA to quantitate DCD in samples. An antibody specific for DCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Proteolysis Inducing Factor/Dermcidin (PIF/DCD) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.