Netrin is a class of protein involved in axon guidance, genetically conserved across C. elegans, Drosophila, frogs, and mice. The genes that encode the first discovered netrins was found to be 50% identical to the gene the unc-6 protein that plays a role in the development of neural systems in nematodes. Netrin-1 is a growth cue which is expressed in neuroepithelial cells in the optic nerve head (ONH). Although it is not required for retinal ganglion cell (RGC) axons to reach the ONH, it does favor their entrance by means of chemoattraction. However, growth of Xenopus RGC's on laminin substrate change the Netrin-1 response to chemorepulsion, which causes the RGC axon to be pushed into the optic nerve head.
Human Netrin-1 (NTN1) ELISA Kit employs a two-site sandwich ELISA to quantitate NTN1 in samples. An antibody specific for NTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NTN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Netrin-1 (NTN1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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