In patients with cancer, symptoms of limbic and brainstem dysfunction may result from a paraneoplastic disorder. Paraneoplastic limbic or brainstem encephalitis occurs more frequently with testicular cancer than with most other cancers.By immunohistochemical and immunoblotting techniques, Voltz et al. (1999) detected antibodies in serum and cerebrospinal fluid against a 40-kD neuronal protein in 10 of 13 patients with testicular cancer and paraneoplastic limbic or brainstem encephalitis, or both. They designated the protein MA2, and found that it was selectively expressed by normal brain tissue and by the testicular tumors of the patients. MA2 shares homology with MA1, a 'brain-testis-cancer' gene related to other neoplastic syndromes and tumors.
Human Paraneoplastic antigen Ma2 (PNMA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PNMA2 in samples. An antibody specific for PNMA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNMA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNMA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNMA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Paraneoplastic antigen Ma2 (PNMA2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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