MUTYH glycosylase, involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene.Mutations in the MUTYH gene cause an autosomal recessive form of familial adenomatous polyposis. Polyps caused by mutated M
Human A/G-specific adenine DNA glycosylase (MUTYH) ELISA Kit employs a two-site sandwich ELISA to quantitate MUTYH in samples. An antibody specific for MUTYH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUTYH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUTYH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUTYH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human A/G-specific adenine DNA glycosylase (MUTYH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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