Human Deleted in azoospermia protein 3 (DAZ3) ELISA Kit,Abbkine_specification/price/image

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DAZ3 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of DAZ3 also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. DAZ3 contains one copy of the 10.8 kb repeat.

Human Deleted in azoospermia protein 3 (DAZ3) ELISA Kit employs a two-site sandwich ELISA to quantitate DAZ3 in samples. An antibody specific for DAZ3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDAZ3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAZ3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAZ3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Deleted in azoospermia protein 3 (DAZ3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.