DLG5 encodes a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. The protein encoded by this gene localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure. Alternative splice variants have been described but their biological nature has not been determined.
Human Disks large homolog 5 (DLG5) ELISA Kit employs a two-site sandwich ELISA to quantitate DLG5 in samples. An antibody specific for DLG5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLG5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLG5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLG5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Disks large homolog 5 (DLG5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-disks-large-homolog-5-dlg5-elisa-kit-kte62026/
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