Dystrophin is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This complex is variously known as the costamere or the dystrophin-associated protein complex. Many muscle proteins, such as α-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with dystrophin at the costamere.The DMD gene, encoding the dystrophin protein, is one of the longest human genes known, covering 2.3 megabases (0.08% of the human genome) at locus Xp21. The primary transcript in muscle measures about 2,100 kilobases and takes 16 hours to transcribe; the mature mRNA measures 14.0 kilobases. The 79-exon muscle transcript[5] codes for a protein of 3685 amino acid residues.
Human Dystrophin (DMD) ELISA Kit employs a two-site sandwich ELISA to quantitate DMD in samples. An antibody specific for DMD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dystrophin (DMD) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-dystrophin-dmd-elisa-kit-kte62014/
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