Endo-S2 is an endo-n-acetylglucosaminase derived from streptococcus pyogenes that excises the glycosidic bond between the two innermost n-acetylglucosamines of the glycoprotein n-linking sugar chain. It is effective for high mannose type, hybrid type and double antenna complex type. Can be made for all human IgG, as well as mice, rats, monkey, goat, sheep, cattle and horse IgG antibody glycosyl.
1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume.
2. Add 1 µl EndoS2.
3. Incubate reaction at 37°C for 1 hour
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl.
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