New UHPLC-MS Workflows Designed for Biopharmaceutical Characterization

Therapeutic protein analysis kits, columns and software among highlights at Pittcon 2016

 

ATLANTA – Pittcon 2016 – (March 7, 2016) – Biopharmaceutical companies can nowachieve advanced levels of specificity, sensitivity and speed with specialized analyticalworkflow solutions for the characterization and quantification of proteins.

 

These new and comprehensive workflow solutions for in-depth biopharmaceuticalcharacterization are being exhibited at Pittcon 2016 (booth 2239) at the Georgia WorldCongress Center, Atlanta.

 

“Characterization and quantitation of biomolecules is challenging and scientists requireuser-friendly instruments and complementary consumables and software to helpthem develop safe and effective drugs faster,” said John Rontree, senior director ofbiopharmaceutical marketing, chromatography and mass spectrometry, Thermo Fisher.“We’ve developed application-based analytical workflows that enable our customers togather more information about novel therapeutics earlier in the research and development process. By offering a complete solution from a single provider, ourcustomers can be confident that their complete characterization is supported by ourglobal service network.”

 

The complete workflows, which include solutions for everything from sample prepto data analysis, feature the following new instruments, consumables and software:

 

· The Thermo Scientific SMART Digest Kit is a protein digestion kit forbiopharmaceutical applications that require reproducible, sensitive and fast,bottom-up analysis of proteins in high- throughput workflows. Designed tominimize preparation time and variability, the SMART Digest kit facilitates fast andreliable analytical results using a simple three-step, easy-to-implement process thatcan also be automated. The immobilized trypsin design is formulated to produce high-quality data with reduced sample preparation time compared to traditional in-solution digestion methods.

 

· The new Thermo Scientific Vanquish Flex UHPLC System is a fully bioinert ultra high performance liquid chromatography (UHPLC) offering that deliversexceptional performance and reliable separations with the flexibility required forlabs developing methods ranging from high- throughput analyses to pharmaceuticalquality control applications. The Vanquish Flex system is designed for high speed,resolution and sensitivity, allowing researchers who perform biopharmaceuticalanalyses to obtain high-quality and consistent data. Because it features integratedmodularity, customers can mix and match modules depending on the demands ofthe application. Biopharmaceutical partners already using the system note itsultra-low baseline noise, retention time reproducibility and peak profilereproducibility.

 

· The Thermo Scientific Dionex Chromeleon 7.2 Chromatography Data System provides a workflow that allows scientists to answer questions that frequently arise during method development.

 

· The Thermo Scientific MAbPac RP column is designed for analytical chemistsrequiring high- resolution separation of intact proteins, such as monoclonal antibody(mAb) variants and antibody drug conjugates (ADCs) as well as those conductinghigh-resolution mass spectrometric analysis of mAbs and mAb fragments. Thiscolumn addresses the increased need for reliable and rapid characterization of thesecompounds required for upstream biopharmaceutical development. The MAbPac RPcolumn is targeted for MS and UV detection: its thermal and pH stability yields promote consistent separations, while the polymeric resin has a pore size suitable formAbs

 

· The new Thermo Scientific BioPharma Finder software is designed toprovide intelligent batch/lot comparisons to promote consistency and qualitythroughout. For intact mass analysis, BioPharma Finder uses two deconvolutionalgorithms to take advantage of the high-quality, high- resolution accurate mass(HRAM) data produced by Thermo Scientific Orbitrap-based mass spectrometers,such as the Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer. For peptide mapping workflows, BioPharma Finder facilitates confirmation of amino acidsequence and identification of the site and type of expected and unexpected post-translational modifications (PTMs), and it provides the relative amount of each modification. Additional features include disulfide bond mapping and low-levelimpurity identification.

 

For a closer look at Thermo Fisher’s new UHPLC-MS workflow solutions forbiopharmaceutical characterization, visit booth 2239 or, for more information, visit www.thermoscientific.com/UHPLC.

 

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science, with revenues of $17billion and more than 50,000 employees in 50 countries. Our mission is to enable ourcustomers to make the world healthier, cleaner and safer. We help our customersaccelerate life sciences research, solve complex analytical challenges, improve patientdiagnostics and increase laboratory productivity. Through our premier brands – ThermoScientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – weoffer an unmatched combination of innovative technologies, purchasing convenienceand comprehensive support. For more information, please visit www.thermofisher.com.