Waters Corporation Brings High Definition to Proteomics at HUPO 2007

Company Showcases Synapt High Definition MS and Identity E High Definition Proteomics Systems

At the Human Proteome Organization's 6 th Annual World Congress, Waters Corporation  is showcasing several technologies expected to give proteomics researchers new insights into the workings of the human proteome.

Waters Synapt™ High Definition Mass Spectometry™ System – Differentiating Ions by Size, Shape, and Charge as Well as Mass

The Waters® Synapt HDMS™ System is intended for researchers working at the limits of conventional mass spectrometry (MS) and who need to further characterize and define their samples.

The Synapt HDMS system is the first mass spectrometer of its kind to employ high efficiency ion-mobility based measurements and separations and specialized software to enable the analysis of sample ions differentiated by size, shape and charge as well as mass. This additional dimension of separations fidelity leads to improved specificity and sample definition, meaning scientists can extract more information about their samples including the detection of previously unseen constituents.

The United Kingdom's Oxford University, Cambridge University, University of Warwick, University of Leeds, and Nottingham University, in addition to Utrecht University, The Netherlands, and the Max Planck Institute, Martinsreid, Germany use the Synapt HDMS System for the study of proteins and to shed light on the causes of some of the world's most pernicious protein-misfolding diseases including Alzheimer's, Parkinson's, Huntington's and "Mad Cow" diseases.

Waters Identity^E High Definition Proteomics System for Label-Free Protein Identification

The Waters® Identity^E High Definition Proteomics™ System is an UltraPerformance LC® (UPLC)/High-Bandwidth Mass Spectrometry (MSE) system solution designed for highly-confident protein identification and to meet stringent requirements as outlined by leading scientific journals.

The Waters Identity^E High Definition Proteomics System catalogs complex protein digest mixtures containing tens of thousands of peptides. The system features a Waters nanoACQUITY® UltraPerformance LC(UPLC®) System for high chromatographic peak capacity and retention time reproducibility, together with an electrospray tandem mass spectrometer - Waters Q-Tof Premier™ or Waters Synapt HDMS - for high dynamic mass resolution and consistent over-sampling of complex protein digests – two hallmarks of high definition proteomics.

HUPO Dinner Forum to Feature Presentations from World Class Scientists

Waters is hosting a dinner forum where invited speakers will present on novel techniques for protein identification. The agenda features scientists from leading European and Korean institutions in the area of protein research.

• Identification, Quantification and Annotation of Heart Membrane Proteins Using Identity^E , Prof. Mike Dunn, UCD, Dublin, Ireland

• Analysis of Post-Translational Modifications by Ion Mobility and Mass Spectrometry , Prof. Ole N. Jensen, University of Southern Denmark

• A Strategy for Comprehensive Identification of Post-translational Modifications in Cellular ProteinsIncluding Low Abundant Modifications, Prof. Kong-Joo Lee, Ewha Womans University, Korea

Waters Poster Presentations

Waters scientists are presenting several posters at HUPO. Copies of the posters will be available for downloading from Waters web site shortly after HUPO concludes.

• Separation and Detection of Protein Post-Translational Modifications by Liquid Chromatography Coupled With a Novel Ion Mobility Mass Spectrometer, Mark Ritchie; Chris Hughes; Therese Mckenna, James Langridge, Waters Corp. MS Technologies Centre, Manchester, United Kingdom

• Top Down Sequence Analysis of Intact Proteins Using Ion Mobility Coupled With Time-of-Flight Mass Spectrometry, Therese McKenna, James Langridge, Christopher J Hughes, Waters Corp. MS Technologies Centre, Manchester, United Kingdom

• High Speed Nanoscale UPLC Separations Combined With Off-line MALDI MS/MS for Peptide Analysis, James I Langridge; Marten Snel, Emmanuelle Claude, Roy Martin, Therese McKenna, Waters Corp. MS Technologies Centre, Manchester, United Kingdom

• Identification, Quantification and Annotation of Heart Membrane Proteins Using Label-Free Nanoscale LC-MS, Pamela M. Donoghue 1, Johannes P.C. Vissers 2, James I. Langridge 2, Michael J. Dunn 3; 1 Immune Regulation Research, Trinity College Dublin, 2 Waters Corporation, MS Technologies Center, Manchester, UK, 3 Proteome Research Centre, UCD Conway Institute, University College Dublin

• Sequencing and Characterization of Human Histones by Ion Mobility Tandem Mass Spectrometry, Ms. Hye Ryung Jung 1, Chris Hughes 2, Therese McKenna 2, James Langridge 2, Prof. Ole N Jensen 1, 1University of Southern Denmark; 2Waters Corporation

About Proteomics

Understanding the role of proteins in disease mechanisms and other biological processes is not an easy undertaking and requires high definition analytical techniques that can unambiguously identify proteins over a very wide dynamic range of concentration in complex biological extracts. Protein identification is the foundation of practical proteomics. Quantifying proteins present in a biological sample and understanding their three-dimension shape and how they interact with each other presents scientists with significant additional challenges.

The first decade of proteomics created high expectations for rapid advances in the health and life sciences. However, the degree of stringency required in data collection/analysis was often underestimated resulting in numerous findings of questionable quality. The systemic under-sampling of traditional LC/MS/MS strategies has contributed significantly to lack of stringency in protein identifications to date

For more information on Waters Synapt High Definition MS System or Waters Identity^E High Definition Proteomics System, visit

www.waters.com/hdms or www.waters.com/identity.

About Waters Corporation

Waters Corporation (NYSE: WAT) creates business advantage for laboratory-dependent organizations by delivering practical and sustainable innovation to enable significant advancements in such areas as healthcare delivery, environmental management, food safety, and water quality worldwide.

Pioneering a connected portfolio of separations science, laboratory information management, mass spectrometry and thermal analysis, supported by an expert customer services team, Waters technology breakthroughs and laboratory solutions provide an enduring platform for customer success.

With revenue of $1.28 billion in 2006 and 4,700 employees, Waters is driving scientific discovery and operational excellence for customers worldwide.