Detection Principle:

This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. Specific anti rat IL-1 & amp; Betathe antibody was pre coated on a high affinity enzyme plate. Add the standard, sample to be tested and biotinylated detection antibody into the wells of the enzyme plate. After incubation, the IL-1 & amp; amp; amp; amp; nbsp; in the sample; Betacombine with solid-phase antibody and detection antibody to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin (streptavidin HRP) was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nmwavelength (reference correction wavelength 540nmor 570nm).

Detection Type: double antibody sandwich method

Form: pre coated 96orifice plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96well plates, standards, anti rat IL-1 & amp; Betaa copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, stop solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.8pg/ml

Detection range: 62.5 - 4000 pg/ml

Recovery range: 82-110%

Storage method: 2-8℃

Background:

Interleukin -1(il-1) protein family members include classical il-1and il-1. il-1 receptor antagonists (il-1ra), il-18, il-33, il-1f5-f10, il-1  and IL-1  have the same cell surface binding receptors and the same biological functions. The unstimulated cells of healthy people do not produce IL-1 , with the exception of skin keratinocytes, some epithelial cells, and some cells of the central nervous system. However, in response to inflammatory factors, infection, or microbial endotoxin, il-1expression in macrophages and other types of cells increased sharplyIL-1  plays an important role in the physiological processes of immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, gh/igf-1 . A variety of diseases are associated with dysregulated or delayed expression of IL-1 , including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, arteriosclerosis, nerve injury and diseases related to senile degeneration.

IL-1and IL-1are structurally related polypeptides with 25% homology at the amino acid level. Both are synthesized into a 31kda precursor and then cleaved into a mature protein of approximately 17.5 kDa . The precursor cleavage of il-1  by caspase-1/ice is a key step in the inflammatory response. Although IL-1  and IL-1  do not contain a typical hydrophobic signal peptide, there is evidence that they can be secreted to the extracellular via non canonical pathways. The untreated il-1fraction can be displayed on the cell membrane and may retain biological activity. Unlike il-1precursors, il-1precursors have little or no biological activity compared with mature il-1precursor and mature il-1are transported to the extracellular space.

IL-1and IL-1exert their effects through binding of immunoglobulin superfamily receptors to IL-1ra the itype transmembrane receptor (il-1ri) of 80 kDa is expressed in a variety of cells, including T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytesII transmembrane receptor (il-1rii) of 68 kDa is expressed in B cells, neutrophils, and bone marrow cellsIL-1RI and il-1rii share 28%homology. But other regions are significantly different: the intracellular domain of type II receptors has only 29 amino acids, while the intracellular domain of type I receptors has 213 amino acidsil-1rii appears to be unresponsive to IL-1 signals, and its function is to act as a decoy receptor to weaken the role of IL-1 IL-1 receptor ligand protein (il-1 racp) and IL-1RI is required for IL-1RI signalingIL-1ra is a secreted molecule and a competitive inhibitor of IL-1 . Soluble IL-1RI and il-1rii exist in human plasma, joint fluid, and conditioned media of several cell lines. In addition, vaccinia virus encodes a IL-1 - binding protein similar to soluble IL-1RI.