Human DNA primase small subunit (PRIM1) ELISA Kit,Abbkine_specification/price/image

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The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. The DNA polymerase-alpha/primase complex contains 4 subunits: the polymerase-alpha p180 (POLA) and p68 (POLA2) subunits, and the primase p58 (PRIM2A) and p49 (PRIM1) subunits. Primase synthesizes oligoribonucleotides that serve as primers for the initiation of DNA synthesis. It plays a role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis

Human DNA primase small subunit (PRIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIM1 in samples. An antibody specific for PRIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human DNA primase small subunit (PRIM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-dna-primase-small-subunit-prim1-elisa-kit-kte61158/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.