IL-1α Precursor encoded by Pro-IL-1a is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. Pro-IL-1a and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
Human IL-1α Precursor (Pro-IL-1a) ELISA Kit employs a two-site sandwich ELISA to quantitate Pro-IL-1a in samples. An antibody specific for Pro-IL-1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPro-IL-1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Pro-IL-1a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Pro-IL-1a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human IL-1α Precursor (Pro-IL-1a) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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