Human 14-3-3 protein sigma (SFN) ELISA Kit,Abbkine_specification/price/image

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Stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Exogenous introduction of 14-3-3-sigma into cycling cells results in a G2 arrest. After DNA damage, these cells initially arrested in the G2 phase of the cell cycle, but unlike cells containing 14-3-3-sigma, the 14-3-3-sigma -/- cells were unable to maintain cell cycle arrest. The 14-3-3-sigma -/- cells died (mitotic catastrophe) as they entered mitosis. This process was associated with a failure of the 14-3-3-sigma-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus.

Human 14-3-3 protein sigma (SFN) ELISA Kit employs a two-site sandwich ELISA to quantitate SFN in samples. An antibody specific for SFN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human 14-3-3 protein sigma (SFN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.