Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF1, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules.The deduced 871-amino acid protein contains an N-terminal signal sequence, followed by a sulfatase domain, a hydrophilic region, and a C-terminal substrate recognition domain. It also has a coiled-coil region, 10 N-glycosylation sites, and several consensus furin cleavage sites. SULF1 shares about 64% identity with SULF2 and 93% identity with mouse Sulf1.
Human Extracellular sulfatase Sulf-1 (SULF1) ELISA Kit employs a two-site sandwich ELISA to quantitate SULF1 in samples. An antibody specific for SULF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySULF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Extracellular sulfatase Sulf-1 (SULF1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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