Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit,Abbkine_specification/price/image

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STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The deduced 547-amino acid protein has a calculated molecular mass of 59.6 kD. STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detected ubiquitous expression of a 3.8-kb transcript, with most abundant expression in brain and pancreas. Western blot analysis of mouse tissues detected a protein of about 60 kD in all tissues examined.

Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit employs a two-site sandwich ELISA to quantitate STK39 in samples. An antibody specific for STK39 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTK39 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STK39 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STK39 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.