The TMX3 gene encodes a protein of 454 amino acid residues that contains a predicted N-terminal signal sequence, a single domain with sequence similarity to thioredoxin and a CGHC active site sequence, a potential transmembrane domain, and a C-terminal KKKD tetrapeptide sequence that matches the classical KKXX-type consensus sequence for ER retrieval of type I transmembrane proteins. Endogenous TMX3 contains endoglycosidase H-sensitive glycans, localizes to the ER by immunofluorescence microscopy, and is present in the membrane fraction after alkaline extraction of the ER luminal content. The TMX3 transcript is found in a variety of tissues and is not up-regulated by the unfolded protein response.
Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX3 in samples. An antibody specific for TMX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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