Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit,Abbkine_specification/price/image

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TMX4, a type I transmembrane protein, was localized to the ER and possessed a Trx-like domain that faced the ER lumen. A maleimide alkylation assay showed that a catalytic CXXC motif in the TMX4 Trx-like domain underwent changes in its redox state depending on cellular redox conditions, and, in the normal state, most of the endogenous TMX4 existed in the oxidized form. The redox potential of this domain indicated that TMX4 could work as a reductase in the environment of the ER. TMX4 had no effect on the acceleration of ER-associated degradation. Because TMX4 interacted with calnexin and ERp57 by co-immunoprecipitation assay, the role of TMX4 may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER.

Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX4 in samples. An antibody specific for TMX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-thioredoxin-related-transmembrane-protein-4-tmx4-elisa-kit-kte60239/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.