Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit,Abbkine_specification/price/image

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VEGF165 is an angiogenic cytokine that also regulates vascular permeability.The neuropilin-1 (np1) receptor binds the 165 amino-acid form of VEGF165 and functions as an enhancer that potentiates VEGF165 signaling via the VEGFR-2 tyrosine-kinase receptor. A VEGF165 mutant (VEGF165KF) that binds to neuropilins but displays a much lower affinity toward VEGFR-1 and VEGFR-2. VEGF165KF failed to induce VEGFR-2 phosphorylation in cells lacking neuropilins. However, in the presence of np1, VEGF165KF bound weakly to VEGFR-2, induced VEGFR-2 phosphorylation, and activated ERK1/2. Interestingly, VEGF165KF did not promote formation of VEGFR-2/np1 complexes nor did high concentrations of VEGF165KF inhibit VEGF165 induced formation of such complexes, suggesting that VEGF165 does not stabilize VEGFR-2/np1 complexes by forming bridges spanning VEGFR-2 and np1.

Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF121 in samples. An antibody specific for VEGF121 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGF121 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF121 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF121 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Vascular Endothelial Growth Factor 121 (VEGF121) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.