VLDL is a type of lipoprotein made by the liver. VLDL is one of the five major groups of lipoproteins (chylomicrons, VLDL, intermediate-density lipoprotein, low-density lipoprotein, high-density lipoprotein) that enable fats and cholesterol to move within the water-based solution of the bloodstream. VLDL is assembled in the liver from cholesterol and apolipoproteins.VLDL is converted in the bloodstream to low-density lipoprotein (LDL). VLDL particles have a diameter of 30-80 nm. VLDL transports endogenous products, whereas chylomicrons transport exogenous (dietary) products.VLDL transports endogenous triglycerides, phospholipids, cholesterol, and cholesteryl esters. It functions as the body's internal transport mechanism for lipids.
Human Very low density lipoprotein (VLDL) ELISA Kit employs a two-site sandwich ELISA to quantitate VLDL in samples. An antibody specific for VLDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVLDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VLDL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VLDL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Very low density lipoprotein (VLDL) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
bio-equip.cn
中文
